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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which leads to chromosome instability, and thus may perhaps contribute to tumorigenesis. This protein was found to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a significant purpose in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have been explained. Two additional splice variants were being noted but detailed nucleotide sequence info is not but available.
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Blocking peptides are peptides that bind particularly towards the goal antibody and block antibody binding. These peptide ordinarily contains the epitope recognized through the antibody.
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This guideline outlines further more possible optimization of mobile and immunoassay parameters to make sure the very best effects are...
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This manual outlines even further probable optimization of cellular and click here immunoassay parameters to guarantee the best possible final results are...
Antibodies sure to the blocking peptide no more bind to the epitope to the concentrate on protein. By evaluating the staining with the blocked antibody compared to the antibody alone, one can see which staining is specific.
Target Info Cyclin E belongs on the very conserved cyclin household, whose users are characterised by a dramatic periodicity in protein abundance in the cell cycle. Cyclins perform as regulators of CDK kinases. Unique cyclins show distinctive expression and degradation designs which contribute for the temporal coordination of each and every mitotic occasion. Cyclin E types a fancy with and capabilities as being a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S transition. This protein accumulates with the G1-S phase boundary and is also degraded as cells progress by S phase.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both of those antibodies...
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